Tallahassee () is the capital city of the U.S. state of Florida. It is the county seat and only incorporated municipality in Leon County. Tallahassee became the capital of Florida, then the Florida Territory, in 1824. In 2010, the population was 181,376, making it the 8th-largest city in the U.S state of Florida, and the 126th-largest city in the United States. The population of the Tallahassee metropolitan area was 385,145 as of 2018. Tallahassee is the largest city in the Florida Big Bend and Florida Panhandle region, and the main center for trade and agriculture in the Florida Big Bend and Southwest Georgia regions. Tallahassee is home to Florida State University, ranked the nation's eighteenth best public university by U.S. News & World Report. It is also home to Florida A&M University, the fifth-largest historically black university by total enrollment. Tallahassee Community College is a large state college that serves mainly as a feeder school to Florida State and Florida A&M. Tallahassee qualifies as a significant college town, with a student population exceeding 70,000.As the capital, Tallahassee is the site of the Florida State Capitol, Supreme Court of Florida, Florida Governor's Mansion, and nearly 30 state agency headquarters. The city is also known for its large number of law firms, lobbying organizations, trade associations and professional associations, including the Florida Bar and the Florida Chamber of Commerce. It is a recognized regional center for scientific research, and home to the National High Magnetic Field Laboratory. In 2015, Tallahassee was awarded the All-American City Award by the National Civic League for the second time.

Albany ( AWL-bə-nee) is a city in the U.S. state of Georgia. Located on the Flint River, it is the seat of Dougherty County, and is the sole incorporated city in that county. Located in southwest Georgia, it is the principal city of the Albany, Georgia metropolitan area. The population was 77,434 at the 2010 U.S. Census, making it the eighth-largest city in the state. It became prominent in the nineteenth century as a shipping and market center, first served by riverboats. Scheduled steamboats connected Albany with the busy port of Apalachicola, Florida. They were replaced by railroads. Seven lines met in Albany, and it was a center of trade in the Southeast. It is part of the Black Belt, the extensive area in the Deep South of cotton plantations. From the mid-20th century, it received military investment during World War II and after, that helped develop the region. Albany and this area were prominent during the civil rights era, particularly during the early 1960s as activists worked to regain voting and other civil rights. Railroad restructuring and reduction in the military here caused job losses, but the city has developed new businesses.

Dothan is a city in Dale, Henry, and Houston counties and the Houston county seat in the U.S. state of Alabama. It is Alabama's eighth-largest city, with a population of 71,072 at the 2020 census. It is near the state's southeastern corner, about 20 miles (32 km) west of Georgia and 16 miles (26 km) north of Florida. It is named after the biblical city where Joseph's brothers threw him into a cistern and sold him into slavery in Egypt.Dothan is the principal city of the Dothan, Alabama metropolitan area, which encompasses all of Geneva, Henry, and Houston counties; the small portion in Dale County is part of the Ozark Micropolitan Statistical Area. The combined population of the entire Dothan metropolitan area in 2010 was 145,639. The city serves as the main transportation and commercial hub for a significant part of southeastern Alabama, southwest Georgia, and nearby portions of the Florida Panhandle. Since approximately one-fourth of the U.S. peanut crop is produced nearby, much of it processed in the city, Dothan is known as "The Peanut Capital of the World". It also hosts the annual National Peanut Festival at the dedicated Peanut Festival Fairgrounds.

Washington County is a county located in the northwestern part of the state of Florida, in the Panhandle. As of the 2010 census, the population was 24,935. Its county seat is Chipley. Washington County is a prohibition or entirely dry county, meaning that the sale of alcoholic beverages is banned in the county.