Iowa is a state in the Midwestern region of the United States, bordered by the Mississippi River to the east and the Missouri River and Big Sioux River to the west. It is bordered by six states: Wisconsin to the northeast, Illinois to the east and southeast, Missouri to the south, Nebraska to the west, South Dakota to the northwest, and Minnesota to the north.

Ames () is a city in Story County, Iowa, United States, located approximately 30 miles (48 km) north of Des Moines in central Iowa. It is best known as the home of Iowa State University (ISU), with leading agriculture, design, engineering, and veterinary medicine colleges. A United States Department of Energy national laboratory, Ames Laboratory, is located on the ISU campus. In 2020, Ames had a population of 66,427. Iowa State University was home to 33,391 students as of fall 2019, which make up approximately one half of the city's population. Ames also hosts United States Department of Agriculture (USDA) sites: the largest federal animal disease center in the United States, USDA's Agricultural Research Service's National Animal Disease Center (NADC), as well as, one of two national USDA sites for the Animal and Plant Health Inspection Service (APHIS), which comprises the National Veterinary Services Laboratory and the Center for Veterinary Biologics. Ames also hosts the headquarters for the Iowa Department of Transportation.

Cedar Falls is a city in Black Hawk County, Iowa, United States. As of the 2020 census, the city population was 40,713. It is home to the University of Northern Iowa, a public university.

Waterloo is a city in and the county seat of Black Hawk County, Iowa, United States. As of the 2020 United States Census the population was 67,314, making it the eighth-largest city in the state. The city is part of the Waterloo – Cedar Falls Metropolitan Statistical Area, and is the more populous of the two cities.