Harrodsburg is a home rule-class city in Mercer County, Kentucky, United States. It is the seat of its county. The population was 8,340 at the 2010 census. Although Harrodsburg was formally established by the House of Burgesses after Boonesborough and was not incorporated by the Kentucky legislature until 1836, it is usually considered the oldest city in Kentucky and has been honored as the oldest permanent American settlement west of the Appalachians.

Nicholasville is a home rule city in and the county seat of Jessamine County, Kentucky, United States. The population was 28,015 during the 2010 U.S. Census, making Nicholasville the 11th-largest settlement in the state. Since the late 20th century, Nicholasville has undergone rapid growth; the population increased 380% between 1970 and 2010. The city serves as both a residential area for Lexington-area commuters and as an employment and shopping center for central Kentucky.

Berea is a home rule-class city in Madison County, Kentucky, in the United States. The town is best known for its art festivals, historic restaurants and buildings, and as the home to Berea College, a private liberal arts college. The population was 13,561 at the 2010 census. It is one of the fastest-growing towns in Kentucky, having increased by 27.4% since 2000. The name appears to come from the King James Bible, being a province in Asia Minor mentioned in Acts 17:11, where its Jewish citizens were more receptive to the message of the Apostles, but also searched the Scriptures each day to check them, in obedience to Isaiah 8:20. Berea is a principal city of the Richmond−Berea Micropolitan Statistical Area, which includes Madison and Rockcastle counties. It was formally incorporated by the state assembly in 1890.