La Crosse is a city in the U.S. state of Wisconsin and the county seat of La Crosse County. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population as of the 2020 was 52,680. The city forms the core of and is the principal city in the La Crosse Metropolitan Area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627.A regional technology, medical, education, manufacturing, and transportation hub, companies based in the La Crosse area include Organic Valley, Logistics Health Incorporated, Kwik Trip, La Crosse Technology, City Brewing Company, and Trane. La Crosse is a college town with over 20,000 students and home to the University of Wisconsin-La Crosse, Viterbo University, and Western Technical College.

Lanesboro is a city in Fillmore County, Minnesota, United States. The population was 754 at the 2010 census. It was named after F. A. Lane, an early landowner. The South Fork of the Root River flows through it, with a waterfall dam across from Sylvan Park. The Root River State Trail and other bike paths also serve the town. An Amish community is in the area and the town is home to many unique shops and restaurants, and a professional stage theater.

Harmony is a city in Fillmore County, Minnesota, United States. The population was 1,020 at the 2010 census. The town bills itself as the "Biggest Little Town in Southern Minnesota" and features the largest Amish community in the state.

Decorah is a city in and the county seat of Winneshiek County, Iowa, United States. The population was 7,587 at the time of the 2020 census. Decorah is located at the intersection of State Highway 9 and U.S. Route 52, and is the largest community in Winneshiek County.