Quincy ( KWIN-see), known as Illinois's "Gem City", is a city in and the county seat of Adams County, Illinois, United States, located on the Mississippi River. The 2010 census counted a population of 40,633 in the city itself, up from 40,366 in 2000. As of July 1, 2015, the Quincy Micro Area had an estimated population of 77,220. During the 19th century, Quincy was a thriving transportation center as riverboats and rail service linked the city to many destinations west and along the river. It was Illinois' second-largest city, surpassing Peoria in 1870. The city has several historic districts, including the Downtown Quincy Historic District and the South Side German Historic District, which display the architecture of Quincy's many German immigrants from the late 19th century.

Keokuk is a city in and a county seat of Lee County, Iowa, United States, along with Fort Madison. It is Iowa's southernmost city. The population was 9,900 at the time of the 2020 census. The city is named after the Sauk chief Keokuk, who is thought to be buried in Rand Park. It is in the extreme southeast corner of Iowa, where the Des Moines River meets the Mississippi. It is at the junction of U.S. Routes 61, 136 and 218. Just across the rivers are the towns of Hamilton and Warsaw, Illinois, and Alexandria, Missouri. Keokuk, along with the city of Fort Madison, is a principal city of the Fort Madison-Keokuk micropolitan area, which includes all of Lee County, Iowa, Hancock County, Illinois and Clark County, Missouri.

Troy is a city in Lincoln County, Missouri, United States. As of the 2010 census the population was 10,540. It is the county seat of Lincoln County.Troy is part of the St. Louis Metropolitan Statistical Area.

Moberly is a city in Randolph County, Missouri, United States. The population was 13,974 at the 2010 census. It is part of the Columbia, Missouri metropolitan area.