Columbus is a city in and the county seat of Platte County, in the state of Nebraska in the Midwestern United States. The population was 22,111 at the 2010 census.

Vermillion (Lakota: Waséoyuze; "The Place Where Vermilion is Obtained") is a city in and the county seat of Clay County. It is located in the southeastern corner of South Dakota, United States and is the eleventh largest city in the state. According to the 2010 Census, the population was 10,571. The city lies atop a bluff near the Missouri River. The area has been home to Native American tribes for centuries. French fur traders first visited in the late 18th century. Vermillion was founded in 1859 and incorporated in 1873. The name refers to the Lakota name: wa sa wak pa'la (red stream). Home to the University of South Dakota, Vermillion has a mixed academic and rural character: the university is a major academic institution for the state, with its only law and medical schools and its only AACSB-accredited business school. Major farm products include corn, soybeans, and alfalfa.

Yankton is a city in and the county seat of Yankton County, South Dakota, United States. Yankton had a population of 14,454 at the 2010 census and it is the principal city of the Yankton Micropolitan Statistical Area, which includes the entirety of Yankton County and which had an estimated population of 22,662 as of July 1, 2017. Yankton was the first capital of Dakota Territory. It is named for the Yankton tribe of Western Dakota people; Yankton is derived from the Dakota word I-hank-ton-wan ("the end village").Yankton is located on the Missouri River just downstream of the Gavins Point Dam and Lewis and Clark Lake, and just upstream of the confluence with the James River. The United States National Park Service's headquarters for the Missouri National Recreational River are located in the city. The Human Services Center was established as a psychiatric hospital in 1882 and is on the National Register of Historic Places. Yankton is commonly referred to as the "River City", due to its proximity to the Missouri River and the importance the river played in the city's settlement and development. Yankton has also earned the nickname "Mother City of the Dakotas", due to the early important role it played in the creation and development of the Dakota Territory, which later became the 39th and 40th U.S. states of North and South Dakota.Owing to the early exploitation of Fort Hays Limestone for cement manufacture, including shipment of cement to the construction of the Panama Canal, Yankton once also had the nickname "Cement City".

South Sioux City is a city in Dakota County, Nebraska, United States. It is located immediately across the Missouri River from Sioux City, Iowa, and is part of the Sioux City, IA-NE-SD Metropolitan Statistical Area. As of the 2010 census, the city population was 13,353, making it the 14th largest city in Nebraska.