Winona is a city in and the county seat of Winona County, in the state of Minnesota. Located in bluff country on the Mississippi River, its most noticeable physical landmark is Sugar Loaf. The city is named after legendary figure Winona, said to have been the first-born daughter of Chief Wapasha (Wabasha) III. The total population of the city was 27,592 at the time of the 2010 census.

Black River Falls is a city in and the county seat of Jackson County, Wisconsin, United States. The population was 3,622 at the 2010 census. It is home to the administrative center of the Ho-Chunk Nation.

La Crosse is a city in the U.S. state of Wisconsin and the county seat of La Crosse County. Positioned alongside the Mississippi River, La Crosse is the largest city on Wisconsin's western border. La Crosse's population as of the 2020 was 52,680. The city forms the core of and is the principal city in the La Crosse Metropolitan Area, which includes all of La Crosse County and Houston County, Minnesota, with a population of 139,627.A regional technology, medical, education, manufacturing, and transportation hub, companies based in the La Crosse area include Organic Valley, Logistics Health Incorporated, Kwik Trip, La Crosse Technology, City Brewing Company, and Trane. La Crosse is a college town with over 20,000 students and home to the University of Wisconsin-La Crosse, Viterbo University, and Western Technical College.

Wabasha is a city in Wabasha County, Minnesota. The population was 2,521 at the time of the 2010 census. Located on the Mississippi River near its confluence with the Chippewa River, it is the county seat.