Jamestown is a city in Stutsman County, North Dakota, United States. It is the county seat of Stutsman County. The population was 15,427 at the 2010 census, making it the ninth largest city in North Dakota. Jamestown was founded in 1872.

Fargo is a city in and the county seat of Cass County, North Dakota, United States. Being the most populous city in the state, it accounts for 16% of the state population. According to the 2020 United States Census estimates, its population was 125,209, making it the 222nd-most populous city in the United States. Fargo, along with its twin city of Moorhead, Minnesota, and the adjacent cities of West Fargo, North Dakota; and Dilworth, Minnesota; form the core of the Fargo-Moorhead, ND-MN Metropolitan Statistical Area. The MSA had a population of 248,591 in 2020. Fargo was founded in 1871 on the Red River of the North floodplain. It is a cultural, retail, health care, educational, and industrial center for southeastern North Dakota and northwestern Minnesota. North Dakota State University is located in the city.

Wahpeton ( WAH-pit-ən) is a city in Richland County, in southeast North Dakota along the Bois de Sioux River at its confluence with the Otter Tail River, which forms the Red River of the North. Wahpeton is the county seat of Richland County. The population was 7,766 at the 2010 census. Wahpeton was founded in 1869 and is the principal city of the Wahpeton Micropolitan Statistical Area, which includes all of Richland County, North Dakota and Wilkin County, Minnesota. Wahpeton's twin city is Breckenridge, Minnesota, on the other side of the river. The Bois de Sioux River and the Otter Tail River join at Wahpeton and Breckenridge to form the Red River of the North. The North Dakota State College of Science is in Wahpeton. The local newspaper is the Wahpeton Daily News.

East Grand Forks (also known as EGF) is a city in Polk County, Minnesota, United States. The population was 8,601 at the 2010 Census, making it the largest community in Polk County.It is located in the Red River Valley region along the eastern bank of the Red River of the North, directly across from the larger city of Grand Forks, North Dakota. The cities of Grand Forks and East Grand Forks form the center of the Grand Forks, ND–MN Metropolitan Statistical Area, which is often called Greater Grand Forks. The metropolitan area had an estimated population of 100,381 on July 1, 2020.