Greenville is the county seat of and the most populous city in Pitt County, North Carolina, United States; the principal city of the Greenville metropolitan area; and the 11th-most populous city in North Carolina. Greenville is the health, entertainment, and educational hub of North Carolina's Tidewater and Coastal Plain. The city's official population as of the 2020 United States Census was 87,521 residents while the Greenville Metropolitan Area includes 170,243 people. In January 2008 and January 2010, Greenville was named one of the nation's "100 Best Communities for Young People" by the America's Promise Alliance. In June 2012, Greenville was ranked in the top ten of the nation's "Best Small Places For Business And Careers" by Forbes magazine. In 2010, Greenville was ranked twenty-fourth in mid-city business growth and development by Forbes Magazine. Greenville is the home of East Carolina University, the fourth-largest university in the University of North Carolina system, and Vidant Medical Center, the flagship hospital for Vidant Health and the teaching hospital for the Brody School of Medicine. The city has the fifth-highest percentage of residents in North Carolina – almost 30 percent – who have obtained bachelor's degrees. At one point, the city became known as "BMX Pro Town USA" as it is home for many top professional BMX riders.

Plymouth is the largest town in Washington County, North Carolina, United States. The population was 3,878 at the 2010 census. It is the county seat of Washington County. Plymouth is located on the Roanoke River about seven miles (11 km) upriver from its mouth into the Albemarle Sound in North Carolina's Inner Banks region.

Washington is a city in Beaufort County, North Carolina, United States, located on the northern bank of the Pamlico River. The population was 9,744 at the 2010 census. It is the county seat of Beaufort County. It is commonly known as "Original Washington" or "Little Washington" to distinguish it from Washington D.C. The closest major city is Greenville, approximately 20 miles (32 km) to the west. Established in 1776 on land donated by Col. James Bonner, Washington is the first city named after George Washington, the first president of the United States.

Edgecombe County is a county located in the U.S. state of North Carolina. As of the 2010 Census, the population was 56,552. Its county seat is Tarboro. Edgecombe County is part of the Rocky Mount, North Carolina, Metropolitan Statistical Area.